know-hows

The REFUGE know-hows : 

  • Bioinformatic searches
  • Preparation of T-DNA vectors (inducible or constitutive overexpression vectors, HpRNAi and amiRNA vectors, reporter gene fusions)
  • High Thoughput production of rice transformants
  • Molecular characterization of the transformants (Q-PCR, northern, Southern, western)
  • Genotyping of insertion lines from local and international collections
  • Growth, crossing and phenotyping (morphology, salinity , drought , (pathogens possible)) in containment greenhouse
  • Allelic variation in diversity panels (core collections)
  • Acces to Cell imaging and Genotyping platforms.