know-hows
The REFUGE know-hows :
- Bioinformatic searches
- Preparation of T-DNA vectors (inducible or constitutive overexpression vectors, HpRNAi and amiRNA vectors, reporter gene fusions)
- High Thoughput production of rice transformants
- Molecular characterization of the transformants (Q-PCR, northern, Southern, western)
- Genotyping of insertion lines from local and international collections
- Growth, crossing and phenotyping (morphology, salinity , drought , (pathogens possible)) in containment greenhouse
- Allelic variation in diversity panels (core collections)
- Acces to Cell imaging and Genotyping platforms.
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